FIGURE

Fig. 6

ID

ZDB-FIG-170725-11

Publication

Ferre-Fernández et al., 2017 - Whole-Exome Sequencing of Congenital Glaucoma Patients Reveals Hypermorphic Variants in GPATCH3, a New Gene Involved in Ocular and Craniofacial Development

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Fig. 6

Immunohistological detection of Gpatch3 in transverse head sections of zebrafish embryos.

(A–D) Expression of gpatch3 was analyzed in 10 μm cryostat sections of fixed zebrafish embryos at 96 hpf by fluorescent immunohistochemistry with a rabbit anti-GPATCH3 primary antibody and a Cy2 donkey anti-rabbit secondary antibody. The green channel corresponds to Gpatch3, the red channel to tissue autofluorescence and the blue channel to DAPI nuclear staining. The arrows indicate mandibular and periocular muscles, the empty arrows indicate jaw cartilages, the white arrowheads indicate periocular mesenchyme, the empty arrowheads indicate corneal endothelium and dermis and the red arrowheads show the corneal epithelium. (C and G) Transmitted light microscopy of panels B and F, respectively. (E–H) Embryos hybridized with only secondary antibody were used as a negative control. Scale bars represent 200 μm in panels A and E and 100 μm in panels (B–D and F–H).

Expression Data

Gene:	gpatch3
Antibody:	Ab1-gpatch3
Fish:	TU
Anatomical Terms:	corneal endothelium extraocular musculature mandibular arch skeleton mandibular muscle
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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