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IL-7R-targeted morpholino oligonucleotides cause abnormal phenotypes and affect thymus development. (A) The expression of IL-7R protein of uninjected (UI), mismatch control (MM), IL-7R-morphant (MO) and IL-7R-rescue (mRNA) groups at 72 hpf. (B) Note that the IL-7R protein expression was significantly suppressed in IL-7R-morphants (ANOVA, *P < 0.05). (C) The gross development of UI, MM, MO and IL-7R-rescue (mRNA) embryos at 72 hpf. Some of the IL-7R-morphants show a slight curve in body axis (arrowhead). (D) The rate of abnormal phenotype increases in MO group (ANOVA, *P < 0.05). (E and F) The images of whole mount in-situ hybridization with mRNA probes recombination activating gene 1 (rag1) and growth hormone1 (gh1) in UI, MM and MO at 5 dpf. E. Note that the expression of rag1 is significantly decreased in IL-7R morphants (E; ANOVA, *P < 0.05, **P < 0.01) while the gh1 positive signals are similar among all three groups (F). Scale bar: C. 500 μm; E. and F., 200 μm.
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