Fig. 12

Drp1 functions with Actr10 in mitochondrial retrograde transport.

(A) EGFP-Actr10 interacts with myc-Drp1 in HEK293T cells. The K38A mutation in Drp1 strengthens this interaction (n = 3 replicates). (B,C) Expression of Drp1^K38A-mRFP causes mitochondrial clustering in wildtype pLL neuron cell bodies at 4 dpf compared to an mRFP only control (B). (D,E) Though mitochondrial morphology is abnormal in actr10 mutant pLL neuron cell bodies (D), expression of Drp1^K38A-mRFP does not cause clustering of this organelle (E). (F–I) While expression of Drp1^K38A-mRFP in wildtype larvae causes loss of mitochondria from the proximal axon compared to mRFP only controls (F,G), mitochondrial number is unaffected by Drp1^K38A-mRFP expression in actr10 mutants (H,I). Arrowheads point to mitochondria in mRFP-expressing axons. Mitochondria are labeled by expression of a mitochondrial targeting sequence tagged with EGFP (5kbneurod:mito-EGFP; green in B-I, white in B'-I'). (J) Quantification of mitochondrial number normalized to mitochondrial size in the proximal axon (ANOVA with post-hoc contrasts; mean ± SEM shown). Scale bars = 10 µm. Number of larvae assayed is indicated on graphs.