ZFIN ID: ZDB-FIG-170602-32
Drerup et al., 2017 - Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 1

actr10 mutants have swollen axon terminals.

(A) Wild-type larva (pigment free) at 4 dpf carrying the neurod:egfp BAC transgene. By 4 dpf, pLL axons are fully extended and functional synapses have formed with hair cells of primary neuromasts. (B) actr10 mutant axons fully extend but terminals display large swellings. Posterior lateral line ganglion (pLLg) and innervation of the third neuromast (NM3) are indicated. Insets in (A) and (B) show magnified NM3 axon terminals. Arrows in inset point to swellings. Asterisks label areas of the pLL nerve obscured by pigment in the mutant. (C) The mutant axon terminal swelling phenotype can be suppressed by exogenous expression of mRFP-Actr10 in a dose-dependent manner. Proportion of mutants with axon terminal swellings is depicted. (D–F) Injection of TALENS targeting exon 1 of the actr10 genomic locus phenocopies the actr10 mutant axon terminal swelling phenotype in F0 injected larvae (arrows). Axons are labeled by the neurod:egfp BAC transgene (white). Scale bars in A,B = 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP actr10nl15/nl15 ; nl1Tg standard conditions Day 4 posterior lateral line nerve axon IFL
Day 4 posterior lateral line nerve axon terminus IFL
Day 4 posterior lateral line neuromast axon terminus IFL
nl1Tg standard conditions Day 4 posterior lateral line nerve axon IFL
Day 4 posterior lateral line nerve axon terminus IFL
Day 4 posterior lateral line neuromast axon terminus IFL
nl1Tg + TALEN1-actr10 standard conditions Day 4 posterior lateral line nerve axon IFL
Day 4 posterior lateral line nerve axon terminus IFL
Day 4 posterior lateral line neuromast axon terminus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
actr10nl15/nl15 ; nl1Tg standard conditions Day 4 posterior lateral line nerve axon terminus swollen, abnormal
Day 4 posterior lateral line neuromast axon terminus swollen, abnormal
nl1Tg + TALEN1-actr10 standard conditions Day 4 posterior lateral line nerve axon terminus swollen, abnormal
Day 4 posterior lateral line neuromast axon terminus swollen, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife