FIGURE

Fig. 3

ID

ZDB-FIG-170525-3

Publication

Suli et al., 2014 - A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations.

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

tUAS is not silenced, as determined by assessing individuals from the F3 generation of the Tg(CV,tUAS:tRFP)^w80 reporter line. Eighteen adults from the F3 generation of Tg(CV,tUAS:tRFP)^w80 were crossed to the F2 generation of Tg(CC,myo6b:nlsTrpR-G4AD)^w83. Progeny double positive for red lenses (indicating the driver line; A, arrow) and green lenses (indicating the reporter line; B, arrow) were sorted out and scored for overall hair cell expression. A single embryo is presented (A, red channel; B, green channel) showing lens expression (A,B) and hair cell expression (A). Of the combined progeny double positive for red and green lens expression, 24.8% (171/690) were also positive for hair cell expression. None was positive for both lens markers but negative for hair cell expression.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development