Fig. 3
tUAS is not silenced, as determined by assessing individuals from the F3 generation of the Tg(CV,tUAS:tRFP)w80 reporter line. Eighteen adults from the F3 generation of Tg(CV,tUAS:tRFP)w80 were crossed to the F2 generation of Tg(CC,myo6b:nlsTrpR-G4AD)w83. Progeny double positive for red lenses (indicating the driver line; A, arrow) and green lenses (indicating the reporter line; B, arrow) were sorted out and scored for overall hair cell expression. A single embryo is presented (A, red channel; B, green channel) showing lens expression (A,B) and hair cell expression (A). Of the combined progeny double positive for red and green lens expression, 24.8% (171/690) were also positive for hair cell expression. None was positive for both lens markers but negative for hair cell expression.