Fig. S4

rs7539120 acts as an in vivo enhancer that overlaps one of the nos1apa endogenous expression domains. A) The rs7539120 non-risk haplotype con­struct, like the risk haplotype (see Figure 2), injected at 1-2 cell stage in developing zebrafish embryos drives transient reporter expression (enhanced GFP; eGFP) in forebrain 24 hours post fertilization in -25% of injected embryos. Representative images from three different embryos with forebrain eGFP expression (white spots, strong expression indicated by yellow arrows) are shown in top row. No eGFP expression was observed from the injections of rs 7539120 deletion ( +/- 5 bases) construct. Representative images from three different embryos with no eGFP expression are shown in bottom row. FB: forebrain; YS: yolk sac. B) (top) A brightfield image of a zebrafish embryo at 24 hours post fertiliza­tion. The red line marks the area used to dissect the head and the remainder of the body for expression anal­ysis. (bottom) RT-PCR on cDNA prepared from either the entire embryos (Whole), or the head region (Head), or the remainder of the body (Remainder), as depicted in image above, using nos1apa and actb1 primers. nos1apa expression was observed in all three samples. actb 1 was used as house-keeping gene control. Control lane represents water samples used as negative con­trol. M: DNA ladder; bp: base pair.