FIGURE

Fig. 7

ID

ZDB-FIG-170314-10

Publication

Williams et al., 2017 - Cyp1b1 Regulates Ocular Fissure Closure Through a Retinoic Acid-Independent Pathway

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Fig. 7

Cyp1b1 differentially regulated vsx2, shh, and pax6. Whole-mount in situ hybridization at 24 hpf demonstrated that vsx2 expression in the eye decreased in response to cyp1b1 overexpression (D, D') compared with Cyp1b1 MO knockdown (C, C'), control-injected (B, B'), and uninjected (A, A'') embryos. Note the appearance of tissue bridging the two optic cups in the cyp1b1 mRNA-injected embryo, as demarcated by vsx2 (arrow). Shha at 24 hpf showed increased expression in the midline floor plate between the eyes in embryos injected with cyp1b1 mRNA (arrowheads, H, H') compared with Cyp1b1 MO knockdown (G, G'), control -injected (F, F'), and uninjected (E, D') embryos. Pax6a and pax6b expression in the retina at 48 hpf decreased in response to cypb1 overexpression (L, L') compared with Cyp1b1 knockdown (K, K'), control-injected (J, J'), and uninjected (I, I') embryos.

Expression Data

Genes:	pax6a pax6b shha vsx2
Fish:	WT WT + MO3-cyp1b1
Knockdown Reagent:	MO3-cyp1b1
Anatomical Terms:	eye floor plate retina
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.