Fig. 4
- ID
- ZDB-FIG-170303-16
- Publication
- Kuri et al., 2017 - A high-sensitivity, bi-directional reporter to monitor NF-?B activity in cell culture and zebrafish in real-time
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Dynamic NF-?B activity during early zebrafish embryonic development. (A?E) Light-sheet images of Tg(8×Hs.NF?B:GFP,Luciferase) embryos show ubiquitous, weak NF-?B activity from 6?hpf until 12?hpf. (F) The first strong tissue-specific signal was observed in the nasal vesicle (nv) at around 16?hpf. (G?K) Confocal images of Tg(8×Hs.NF?B:GFP,Luciferase) embryos from 1?dpf until 5?dpf. GFP was detected in the proctodeum (p) at 1?dpf (G). The GFP signal in the nasal vesicle (G) originates from microvillous sensory neurons (H). E?G and I?K show embryos in lateral view. Anterior is to the left. H shows the head of embryos from the frontal side. Yellow arrows indicate GFP in the lateral line. White arrowheads indicate GFP in the intestine. Scale bars: 100?µm (A?F); 300?µm (G); 50?µm (H); 500?µm (I?K). |