(A) Labeled and dimensioned enface SolidWorks drawing of the zWEDGI mold. (B) SolidWorks isometric of mold and PDMS device. Upper expanded view details the structure of the restraining tunnel and lower view shows positioning of larva. All dimensions are in mm unless specifically noted. (C) Flow chart illustrating procedure for making zWEDGI. Details of the procedure are in the Materials and Methods section. (D) Finished PDMS device plasma bonded to glass bottom dish and a detailed view of a single channel with compartments labeled. Scale bar?=?1?mm. PDMS, polydimethylsiloxane; zWEDGI, zebrafish Wounding and Entrapment Device for Growth and Imaging.
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