Fig. 2

Spatial control of TAEL induction achieved using four different imaging modalities. Left column shows activation region (blue) for each method. Middle and right columns show resulting mCherry expression (red) 4?h post-activation. (A,C,E,G,I,K) Bright-field and 488?nm channels merged. (B,D,F,H,J,L) Bright-field and 561?nm channels merged. (B?,D?,F?,H?,J?,L?) 561?nm channel only. Unless otherwise noted, images are lateral views with rostral to the left. (A-B?) Closing down the field diaphragm on an epifluorescence microscope (488?nm, GFP excitation setting) restricts the light coming through the objective and illuminates the sample with a small hexagonal column. (C-D?) Region of interest (ROI) on a point scanning confocal to restrict scanning of the 488?nm laser to a small square. (E-H?) Digital micromirror device (DMD) illuminated with a 470?nm LED to project variously sized square columns of blue light onto the embryo. (I-L?) Restricted scanning range of the 488?nm laser on a digital scanned laser light sheet microscope (DSLM) to project variously wide beams of blue light through the embryo. (I) En face view with head and tail as indicated. (J-J?) Dorsal view with anterior to the bottom. Brackets (L,L?) indicate an example of ?off-target? mCherry expression. Arrows in I and K indicate position of the light sheet. Scale bars: 100?µm.