FIGURE

Fig. 6

ID

ZDB-FIG-170210-6

Publication

Wang et al., 2016 - The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Maeg regulates angiogenesis dependent on RGD domain. A. The diagram of Maeg protein with RGD domain and Maeg protein with RGD domain mutated to RGE domain (Maeg^ΔRGD). B. Confocal images of ISVs in control embryos, maeg morphants, maeg morphants treated with DAPT, and maeg morphants coinjected with dll4 MO using Tg(kdrl:EGFP) transgenic embryos. C. Percentage of embryos with ISV defect in each group. χ² test; ***, P<0.001. D, E. Phosphorylation levels of ERK and Akt determined by Western blot analysis in maeg loss- and gain-of-function embryos. F. Confocal images of ISVs in control embryos, maeg mutants, itgb1a morphants, and itgb1a morphants coinjected with maeg mRNA using Tg(kdrl:EGFP) transgenic embryos. G. Percentage of embryos with ISV defect in each group.

Expression Data

Gene:	EGFP
Antibodies:	Ab11-mapk Ab20-mapk
Fish:	egfl6^zf774/zf774 (AB) s843Tg s843Tg + MO1-itgb1a egfl6^zf774/zf774; s843Tg
Knockdown Reagent:	MO1-itgb1a
Anatomical Terms:	trunk vasculature whole organism
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	egfl6^zf774/zf774 (AB) s843Tg + MO1-itgb1a egfl6^zf774/zf774; s843Tg
Knockdown Reagent:	MO1-itgb1a
Observed In:	dorsal longitudinal anastomotic vessel blood vessel morphogenesis intersegmental vessel sprouting angiogenesis whole organism
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncotarget