FIGURE

Fig. 9

ID

ZDB-FIG-170208-45

Publication

Grampa et al., 2016 - Novel NEK8 Mutations Cause Severe Syndromic Renal Cystic Dysplasia through YAP Dysregulation

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Fig. 9

NEK8 overexpression in zebrafish induces Hippo pathway deregulation.

(A) Representative pictures of the different classes of body phenotype observed upon nek8 morpholino (MO) or human NEK8-GFP RNA injections in zebrafish embryos. Arrowhead points out cyclopia observed in a subset of short embryos. (A') Graph showing the distribution of the three classes of body axis curvature observed in rescue experiments with WT or mutated (T87A, R602W) NEK8-GFP RNA forms. 30% of embryos co-injected with nek8 MO and WT NEK8-GFP RNA exhibited a stunted dorsally curved body axis similar to embryos injected with human YAP RNA. Data are from three independent experiments (n = 90 to 180 embryos). (A'') Graph showing the distribution of normal and stunted dorsally curved animals among GFP or WT NEK8-GFP RNA injected embryos upon Verteporfin (VP) treatment. A partial rescue of the phenotype can be observed in the presence of 20 μM Verteporfin. Data are from four independent experiments (n = 120 to 240 embryos).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-nek8
Knockdown Reagent:	MO3-nek8
Observed In:	whole organism anterior-posterior axis
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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