ZFIN Image: Grampa et al., 2016, Fig. 9

Image

Figure Caption

Fig. 9

NEK8 overexpression in zebrafish induces Hippo pathway deregulation.

(A) Representative pictures of the different classes of body phenotype observed upon nek8 morpholino (MO) or human NEK8-GFP RNA injections in zebrafish embryos. Arrowhead points out cyclopia observed in a subset of short embryos. (A') Graph showing the distribution of the three classes of body axis curvature observed in rescue experiments with WT or mutated (T87A, R602W) NEK8-GFP RNA forms. 30% of embryos co-injected with nek8 MO and WT NEK8-GFP RNA exhibited a stunted dorsally curved body axis similar to embryos injected with human YAP RNA. Data are from three independent experiments (n = 90 to 180 embryos). (A'') Graph showing the distribution of normal and stunted dorsally curved animals among GFP or WT NEK8-GFP RNA injected embryos upon Verteporfin (VP) treatment. A partial rescue of the phenotype can be observed in the presence of 20 μM Verteporfin. Data are from four independent experiments (n = 120 to 240 embryos).

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.