Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-170208-13
Bielczyk-Maczyńska et al., 2015 - The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish. PLoS Genetics   11:e1005677 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 5

nol9sa1022/sa1022 mutants show a decrease in proliferation of c-myb+ cells.

(A) Representative maximum projection confocal images showing HSPCs (GFPdim, arrow) in the CHT of Tg(cd41:EGFP) larvae at 96 hpf. Larvae are oriented with anterior to the left and dorsal to the top. (B) The number of GFPdim HSPCs observed in the CHT region of nol9sa1022/sa1022 mutants (n = 20) and their wt siblings (n = 51) at 96 hpf. Data are represented as the mean +/- SEM, Student’s t-test, **, p<0.01. (C) Representative confocal images of the CHT of Tg(cmyb:EGFP) embryos subjected to BrdU incorporation assay at 48 hpf. Arrowheads mark double positive BrdU+ c-myb+ cells. Bright field image of a 48 hpf embryo shows the imaged part of the CHT. (D) The total number of c-myb+ cells observed in the CHT of nol9sa1022/sa1022 embryos (n = 13) and their wt siblings (n = 14) subjected to the BrdU incorporation assay at 48 hpf. Data are represented as the mean +/- SEM. Student’s t-test. ns—not significant. (E) The percentage of BrdU+ cells within the c-myb+ population in the CHT of nol9sa1022/sa1022 embryos (n = 13) and their wt siblings (n = 14) subjected to the BrdU incorporation assay at 48 hpf. Data are represented as the mean +/- SEM, Student’s t-test, *, p<0.05.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg ; nol9sa1022/sa1022 standard conditions Day 4 post-vent region hematopoietic multipotent progenitor cell decreased amount, abnormal
nol9sa1022/sa1022; zf169Tg standard conditions Long-pec hematopoietic multipotent progenitor cell cell population proliferation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.