FIGURE

Fig. 6

ID

ZDB-FIG-170130-19

Publication

Müller-Deile et al., 2016 - Overexpression of TGF-β Inducible microRNA-143 in Zebrafish Leads to Impairment of the Glomerular Filtration Barrier by Targeting Proteoglycans

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Fig. 6

Glomerular damage after miR-143 overexpression and knockdown of syndecan and versican in zebrafish larvae. (A) Transmission electron microscopy pictures of the pronephros of zebrafish larvae at 120 hpf after injection with a syndecan 3 morpholino (sdc3-MO, 100 μM), syndecan 4 morpholino (sdc4-MO, 100 μM), versican morpholino (vcan-MO, 100 μM) or control morpholino (CTRL-MO, 100 μM), as well miR- 143 mimic (miR-143, 5 μM) or miR control (miR-CTRL, 5 μM). White arrows illustrate podocyte effacement. Black arrows illustrates loss of endothelial cell fenestration. (Aa) Normal, partial and complete effacement of podocytes was quantified in the different groups. (Ab) Quantification endothelial cell fenestration per μm GBM. *** p<0.001, n.s. not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-vcana AB + MO2-sdc3 AB + MO4-sdc4
Knockdown Reagents:	MO1-vcana MO2-sdc3 MO4-sdc4
Observed In:	glomerular basement membrane glomerular endothelium fenestra pronephric glomerulus pronephric podocyte
Stage:	Day 5

Phenotype Detail

Acknowledgments

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