ZFIN ID: ZDB-FIG-170123-32
Drummond et al., 2017 - The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish. Developmental Biology   421(1):52-66 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 9

Notch signaling also inhibits CS differentiation, but in an upstream or independent way than tbx2a/b. NICD embryos were heat-shocked at 15 ss to ectopically activate Notch signaling and fixed at 24 hpf. Post heat-shock induction, NICD embryos exhibited a dramatic reduction of the CS, while siblings showed normal CS clusters. To block Notch signaling, wild-type embryos were incubated in 100 ┬Ám DAPT/E3 solution from 90% epiboly to the 24 hpf stage. DAPT treatment resulted in a significant elevation in the number of CS cells. Heat-shocked NICD tbx2a/b morphants exhibited a significant expansion in the CS that was in contrast to the reduced CS observed in uninjected heat-shocked NICD siblings. The number of cells in the these CS clusters was not significantly different to the expansion seen in wild-type embryos injected with tbx2a/b MO. DAPT-treated tbx2a/b morphants and fbyc144 mutants exhibited an expansion in the CS that was not significantly different than wild-type embryos treated with DAPT alone. Together, these results suggest that tbx2a/b are acting in the same pathway, downstream of Notch to suppress the CS. Representative images were based on thorough examination of at least 15 embryos per treatment. (*p<0.05, **p<0.001, N.S.=not significant)..

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
stc1 TU standard conditions Prim-5 corpuscles of Stannius ISH
TU chemical treatment by environment: DAPT Prim-5 corpuscles of Stannius ISH
TU + MO1-tbx2 standard conditions Prim-5 corpuscles of Stannius ISH
TU + MO1-tbx2 chemical treatment by environment: DAPT Prim-5 corpuscles of Stannius ISH
kca3Tg; kca4Tg heat shock Prim-5 corpuscles of Stannius ISH
kca3Tg; kca4Tg + MO1-tbx2 heat shock Prim-5 corpuscles of Stannius ISH
tbx2bc144/c144 standard conditions Prim-5 corpuscles of Stannius ISH
tbx2bc144/c144 chemical treatment by environment: DAPT Prim-5 corpuscles of Stannius ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment by environment: DAPT Prim-5 corpuscles of Stannius stc1 expression increased distribution, abnormal
Prim-5 corpuscles of Stannius cell increased amount, abnormal
TU + MO1-tbx2 standard conditions Prim-5 corpuscles of Stannius stc1 expression increased distribution, abnormal
Prim-5 corpuscles of Stannius cell increased amount, abnormal
TU + MO1-tbx2 chemical treatment by environment: DAPT Prim-5 corpuscles of Stannius stc1 expression increased distribution, abnormal
Prim-5 corpuscles of Stannius cell increased amount, abnormal
kca3Tg; kca4Tg heat shock Prim-5 corpuscles of Stannius stc1 expression decreased distribution, abnormal
Prim-5 corpuscles of Stannius cell decreased amount, abnormal
kca3Tg; kca4Tg + MO1-tbx2 heat shock Prim-5 corpuscles of Stannius stc1 expression increased distribution, abnormal
Prim-5 corpuscles of Stannius cell increased amount, abnormal
tbx2bc144/c144 standard conditions Prim-5 corpuscles of Stannius stc1 expression increased distribution, abnormal
Prim-5 corpuscles of Stannius cell increased amount, abnormal
tbx2bc144/c144 chemical treatment by environment: DAPT Prim-5 corpuscles of Stannius stc1 expression increased distribution, abnormal
Prim-5 corpuscles of Stannius cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 421(1), Drummond, B.E., Li, Y., Marra, A.N., Cheng, C.N., Wingert, R.A., The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish, 52-66, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.