Fig. 7

Lipid absorption is decreased in gsp/myoVb mutant larvae.

Bright field images (A–D) of wild type (A, B) and mutant larvae (C, D), either unfed (A) or fed with coloured emulsified yolk (B–D). Electron micrograph of enterocytes from wild type siblings (E, G, H) and gsp/myoVb mutant larvae (F, I, J) fed with liquid yolk (E, F) and solid yolk (low magnification G, I; higher magnification H, J). Histological sections of the anterior gut of well-fed wild-type sibling (K) and gsp/myoVb (L) larvae. The micrographs of liquid yolk fed larval enterocytes were taken in the anterior part of the gut whereas the solid yolk fed larval enterocytes were imaged in the midgut. Note that while the absorption is low in the anterior gut in gsp/myoVb mutant larvae, it is almost absent in the midgut. The EM analysis was done on 3 wild type siblings and 3 mutants out of 9 animals of each genotype, which were additionally analysed by histology (not shown). In the second set, 3 well-fed siblings and 4 well-fed mutants were preselected and used for histological analysis. The representative images for this set are presented in K and L. The black arrowheads point towards the lipid droplets in the enterocytes. Scale bars: in E, F, K and L corresponds to 10 μm, in G, H, I, and J correspond to 2 μm.