FIGURE

Fig. 7

ID

ZDB-FIG-170104-14

Publication

Jiménez-Amilburu et al., 2016 - In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation

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Fig. 7

Blocking Notch Activation Did Not Increase the Number of CMs Exhibiting Apical Constriction or of CMs in the Trabecular Layer

(A–B') Confocal images (mid-sagittal sections) of 81-hpf Tg(myl7:MKATE-CAAX);Tg(TP1:VenusPEST) zebrafish hearts. DMSO-treated larvae show Notch activity in CMs (A and A', asterisks) and atrioventricular canal (AVC) cells (A and A', yellow arrows). Larvae treated with 100 μM DAPT lack Notch activity in both CMs and AVC cells (B and B', Ø).

(C–D') Confocal images (mid-sagittal sections) of 79-hpf Tg(−0.2myl7:EGFP-podxl);Tg(myl7:MKATE-CAAX);Tg(TP1:H2B-mCherry) zebrafish hearts. DMSO-treated larvae show CM apical constrictions (C and C', arrowheads). 79-hpf larvae treated at 48 hpf with 100 μM DAPT show similar numbers of CM apical constrictions than controls (D and D', arrowheads).

(E and F) Number of CM apical constrictions per ventricle at the mid-sagittal plane after 100 μM DAPT treatment. Animals were treated at 48 hpf and imaged at 79 hpf (E) or treated at 60 hpf and imaged at 72 hpf (F). Each dot represents one heart. Data are shown as mean ± SEM. ns, no significant differences by Student’s t test.

(G–H') Confocal images (mid-sagittal sections) of 79-hpf Tg(−0.2myl7:EGFP-podxl);Tg(myl7:MKATE-CAAX);Tg(myl7:nDsRed2) zebrafish hearts. DMSO-treated larvae show normal CM shape in the compact layer (G', arrowheads), while DAPT-treated larvae show abnormal CM shape (H', arrowheads).

(I and J) Graphs showing the percentage of trabecular CMs versus the total number of CMs counted in two regions of the outer curvature. Each dot represents one heart.

Data are shown as mean ± SEM. ^∗p < 0.05 and ^∗∗∗p < 0.001 by Student’s t test. V, ventricle; AVC, atrioventricular canal. Scale bars, 20 μm.

Expression Data

Genes:	DsRed2 EGFP mCherry mKate2 Venus
Fish:	s940Tg; sd11Tg bns103Tg; f2Tg; sd11Tg bns103Tg; s939Tg; sd11Tg
Condition:	chemical treatment: DAPT
Anatomical Terms:	atrioventricular valve cardiac muscle cell cardiac muscle cell apical plasma membrane cardiac muscle cell membrane cardiac muscle cell nucleus
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s940Tg; sd11Tg bns103Tg; f2Tg; sd11Tg
Condition:	chemical treatment: DAPT
Observed In:	atrioventricular valve cardiac muscle cell trabecular layer cardiac muscle cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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