PUBLICATION

In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation

Authors
Jiménez-Amilburu, V., Rasouli, S.J., Staudt, D.W., Nakajima, H., Chiba, A., Mochizuki, N., Stainier, D.Y.
ID
ZDB-PUB-161208-1
Date
2016
Source
Cell Reports   17: 2687-2699 (Journal)
Registered Authors
Mochizuki, Naoki, Nakajima, Hiroyuki, Stainier, Didier, Staudt, David
Keywords
EMT, apical constriction, apicobasal polarity, cardiomyocyte depolarization, live imaging, trabeculation, zebrafish
MeSH Terms
  • Zebrafish/genetics
  • Morphogenesis/genetics
  • Animals, Genetically Modified
  • Cell Polarity/genetics
  • Neuregulin-1/genetics*
  • Molecular Imaging*
  • Signal Transduction
  • Humans
  • Myocytes, Cardiac/metabolism*
  • Myocytes, Cardiac/pathology
  • Myocytes, Cardiac/ultrastructure
  • Receptors, Notch/genetics
  • Animals
  • Epithelial-Mesenchymal Transition/genetics*
  • Organogenesis/genetics
  • Zebrafish Proteins/genetics
  • Transgenes
  • Myocardial Contraction/genetics*
(all 18)
PubMed
27926871 Full text @ Cell Rep.
Abstract
Despite great strides in understanding cardiac trabeculation, many mechanistic aspects remain unclear. To elucidate how cardiomyocyte shape changes are regulated during this process, we engineered transgenes to label their apical and basolateral membranes. Using these tools, we observed that compact-layer cardiomyocytes are clearly polarized while delaminating cardiomyocytes have lost their polarity. The apical transgene also enabled the imaging of cardiomyocyte apical constriction in real time. Furthermore, we found that Neuregulin signaling and blood flow/cardiac contractility are required for cardiomyocyte apical constriction and depolarization. Notably, we observed the activation of Notch signaling in cardiomyocytes adjacent to those undergoing apical constriction, and we showed that this activation is positively regulated by Neuregulin signaling. Inhibition of Notch signaling did not increase the percentage of cardiomyocytes undergoing apical constriction or of trabecular cardiomyocytes. These studies provide information about cardiomyocyte polarization and enhance our understanding of the complex mechanisms underlying ventricular morphogenesis and maturation.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment: PD 168393Day 4
WTchemical treatment: PD 168393Day 4
WTchemical treatment: PD 168393Day 4
WTchemical treatment: PD 168393Day 4
bns103Tg; f2Tg; sd11Tgchemical treatment: DAPTProtruding-mouth
bns103Tg; f2Tg; sd11Tgchemical treatment: DAPTProtruding-mouth
bns103Tg; sd11Tgchemical treatment: PD 168393Day 4
bns103Tg; sd11Tgchemical treatment: PD 168393Day 4
bns103Tg; sd11Tgchemical treatment: PD 168393Day 4
nrg2amn0237Gt/mn0237Gt; bns103TgcontrolProtruding-mouth
1 - 10 of 20
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Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
tnnt2aMO1-tnnt2aMRPHLNO
1 - 1 of 1
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Fish
Fish
bns103Tg
bns103Tg; f2Tg; sd11Tg
bns103Tg; s939Tg; sd11Tg
bns103Tg; sd11Tg
bns103Tg; sd11Tg + MO1-tnnt2a
cz1701Tg; s959Tg
nrg2amn0237Gt/mn0237Gt; bns103Tg
nrg2amn0237Gt/mn0237Gt; s940Tg
s738Tg; s959Tg
s928Tg; s959Tg
1 - 10 of 15
Show
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
CreEFGCre
DsRedEFGDsRed
DsRed2EFGDsRed2
EGFPEFGEGFP
GFPEFGGFP
mCherryEFGmCherry
mKate2EFGmKate2
mRFP1EFGmRFP1
VenusEFGVenus
1 - 9 of 9
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Mapping
No data available
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Citation
Jiménez-Amilburu, V., Rasouli, S.J., Staudt, D.W., Nakajima, H., Chiba, A., Mochizuki, N., Stainier, D.Y. (2016) In Vivo Visualization of Cardiomyocyte Apicobasal Polarity Reveals Epithelial to Mesenchymal-like Transition during Cardiac Trabeculation. Cell Reports. 17:2687-2699.