FIGURE

Fig. 7

ID

ZDB-FIG-161208-11

Publication

Topczewska et al., 2016 - The Morphogenesis of Cranial Sutures in Zebrafish

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Fig. 7

RNAscope in situ hybridization and IHC for GFP performed for adults at 12 wpf.

(A-F) Sequential sections (4 μm) of interfrontal suture expression. Names of genes tested by RNAscope in situ hybridization are labeled on each image. (A, B) Representative images of the skull taken at low magnification (5x) that visualize entire expression pattern of (A) col1a1a and (B) col10a1a. (C-F) Images of interfrontal sutures and the expression of representative genes as labeled on each image. (G) Immunohistochemistry for sp7 observed in the interfrontal suture using a GFP reporter in the Tg(sp7:EGFP)^b1212 transgenic line. Transverse paraffin section, GFP positive cells (green) and nuclei stained with DAPI (blue).

Expression Data

Genes:	bglap col1a1a col2a1a col10a1a EGFP
Antibody:	Ab12-GFP
Fish:	AB b1212Tg
Anatomical Terms:	inter-frontal joint mesenchymal cell osteoblast
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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