ZFIN ID: ZDB-FIG-161208-11
Topczewska et al., 2016 - The Morphogenesis of Cranial Sutures in Zebrafish. PLoS One   11:e0165775 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 7

RNAscope in situ hybridization and IHC for GFP performed for adults at 12 wpf.

(A-F) Sequential sections (4 μm) of interfrontal suture expression. Names of genes tested by RNAscope in situ hybridization are labeled on each image. (A, B) Representative images of the skull taken at low magnification (5x) that visualize entire expression pattern of (A) col1a1a and (B) col10a1a. (C-F) Images of interfrontal sutures and the expression of representative genes as labeled on each image. (G) Immunohistochemistry for sp7 observed in the interfrontal suture using a GFP reporter in the Tg(sp7:EGFP)b1212 transgenic line. Transverse paraffin section, GFP positive cells (green) and nuclei stained with DAPI (blue).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bglap AB standard conditions Adult inter-frontal joint ISH
Adult osteoblast ISH
col1a1a AB standard conditions Adult inter-frontal joint ISH
Adult mesenchymal cell ISH
Adult osteoblast ISH
col2a1a AB standard conditions Adult Not Detected inter-frontal joint ISH
col10a1a AB standard conditions Adult inter-frontal joint ISH
Adult osteoblast ISH
EGFP Ab12-GFP b1212Tg standard conditions Adult inter-frontal joint IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One