ZFIN ID: ZDB-FIG-161116-17
Almeida et al., 2016 - Oligodendrocyte Development in the Absence of Their Target Axons In Vivo. PLoS One   11:e0164432 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Pec-fin to Protruding-mouth

Fig. 9

Impaired survival of a subset of oligodendrocyte-lineage cells in the posterior spinal cord of kbp mutants.

A, High-magnification live-imaging of the posterior spinal cord of 72hpf Tg(sox10:mRFP) larvae, with Mauthner axon myelin sheaths in WTs (pink bracket) and mRFP+ debris in mutants (pink arrowheads). Scale bars: 10 μm B, Posterior spinal cord of 72hpf Tg(sox10:mRFP) larvae stained with Acridine Orange; mutants have AO+ foci (arrowheads). See text for details. Scale bar: 20 μm. C, Time-lapse stills showing a mutant oligodendrocyte forming a myelin sheath (brackets) and then dying, originating mRFP+ debris (arrowheads). Scale bar: 10μm. D, Proportion of larvae with oligodendrocyte death events: essentially only mutants show any events, only in the posterior spinal cord. E, Rate of oligodendrocyte (OL) death (per 12-hour) is significantly elevated only in the posterior spinal cord of kbp mutants. Data from N = 18 WT and N = 14 mutants (anterior) and N = 18WT and N = 21 mutants (posterior). * indicates P<0.05; see text for details. Error bars indicate ± SD.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP kifbpst23/st23; vu12Tg; vu234Tg standard conditions Pec-fin oligodendrocyte cytoplasm IFL
vu12Tg; vu234Tg standard conditions Pec-fin oligodendrocyte cytoplasm IFL
mCherry kifbpst23/st23; vu12Tg; vu234Tg standard conditions Pec-fin oligodendrocyte membrane IFL
kifbpst23/st23; vu234Tg standard conditions Protruding-mouth Mauthner neuron myelin sheath IFL
Protruding-mouth oligodendrocyte membrane IFL
vu12Tg; vu234Tg standard conditions Pec-fin oligodendrocyte membrane IFL
vu234Tg standard conditions Protruding-mouth oligodendrocyte membrane IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kifbpst23/st23; vu12Tg; vu234Tg standard conditions Pec-fin oligodendrocyte cell death increased occurrence, abnormal
kifbpst23/st23; vu234Tg standard conditions Protruding-mouth oligodendrocyte cell death increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One