ZFIN ID: ZDB-FIG-161101-4
Poon et al., 2016 - Development of the cardiac conduction system in zebrafish. Gene expression patterns : GEP   21(2):89-96 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

Loss of endocardium and hemodynamic stimulation affects development of the CCS. A: 3 dpf CCS:clo sibling. B: 3 dpf CCS:clom39-/- mutant. SAN CCS cells (arrows) spread widely in clom39-/-. C, E: Control CCS embryos at 2 dpf and 3 dpf, respectively. D, F: tnnt2 morphants at 2 dpf and 3 dpf, respectively. E′, F′: The same embryos as in E and F, showing dsRed expression of CCS:Tg(myl7:dsRed). Dotted white lines outline the chambers of the embryonic zebrafish heart. A-D: lateral view; E-H: ventral view. Abbreviations: A, atrium; SAN, sino-atrial region; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 f2Tg; sqet33mi28Et + MO1-tnnt2a standard conditions Protruding-mouth myocardium IFL
f2Tg; sqet33mi59BEt standard conditions Protruding-mouth myocardium IFL
f2Tg; sqet33mi59BEt + MO1-tnnt2a standard conditions Protruding-mouth myocardium IFL
npas4ls5/s5; sqet33mi28Et standard conditions Protruding-mouth myocardium IFL
EGFP f2Tg; sqet33mi28Et standard conditions Long-pec sinoatrial ring IFL
f2Tg; sqet33mi28Et + MO1-tnnt2a standard conditions Long-pec sinoatrial ring IFL
Protruding-mouth sinoatrial node IFL
f2Tg; sqet33mi59BEt standard conditions Long-pec sinoatrial ring IFL
f2Tg; sqet33mi59BEt + MO1-tnnt2a standard conditions Long-pec sinoatrial ring IFL
Protruding-mouth sinoatrial node IFL
npas4ls5/s5; sqet33mi28Et standard conditions Protruding-mouth sinoatrial node IFL
npas4ls5/s5; sqet33mi59BEt standard conditions Protruding-mouth sinoatrial node IFL
sqet33mi28Et standard conditions Protruding-mouth sinoatrial node IFL
sqet33mi59BEt standard conditions Protruding-mouth sinoatrial node IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
f2Tg; sqet33mi28Et + MO1-tnnt2a standard conditions Long-pec cardiac conduction system position, normal
Long-pec sinoatrial node position, normal
Protruding-mouth cardiac conduction system mislocalised, abnormal
Protruding-mouth sinoatrial node EGFP expression increased distribution, abnormal
Protruding-mouth sinoatrial node increased area, abnormal
f2Tg; sqet33mi59BEt + MO1-tnnt2a standard conditions Long-pec cardiac conduction system position, normal
Long-pec sinoatrial node position, normal
Protruding-mouth cardiac conduction system mislocalised, abnormal
Protruding-mouth sinoatrial node EGFP expression increased distribution, abnormal
Protruding-mouth sinoatrial node increased area, abnormal
npas4ls5/s5; sqet33mi28Et standard conditions Protruding-mouth cardiac conduction system mislocalised, abnormal
Protruding-mouth sinoatrial node EGFP expression increased distribution, abnormal
Protruding-mouth sinoatrial node increased area, abnormal
npas4ls5/s5; sqet33mi59BEt standard conditions Protruding-mouth cardiac conduction system mislocalised, abnormal
Protruding-mouth sinoatrial node EGFP expression increased distribution, abnormal
Protruding-mouth sinoatrial node increased area, abnormal
Acknowledgments:
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Reprinted from Gene expression patterns : GEP, 21(2), Poon, K.L., Liebling, M., Kondrychyn, I., Brand, T., Korzh, V., Development of the cardiac conduction system in zebrafish, 89-96, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns