ZFIN Figure: Poon et al., 2016, Fig. 4

ZFIN ID: ZDB-FIG-161101-4

ZFIN ID: ZDB-FIG-161101-4

Poon et al., 2016 - Development of the cardiac conduction system in zebrafish. Gene expression patterns : GEP 21(2):89-96 Full text @ Gene Expr. Patterns

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	DsRed2 EGFP
Fish:	sqet33mi28Et sqet33mi59BEt sqet33mi28Et ; f2Tg sqet33mi59BEt ; f2Tg sqet33mi28Et ; f2Tg + MO1-tnnt2a sqet33mi28Et ; npas4l^s5/s5 sqet33mi59BEt ; f2Tg + MO1-tnnt2a sqet33mi59BEt ; npas4l^s5/s5
Knockdown Reagent:	MO1-tnnt2a
Anatomical Terms:	myocardium sinoatrial node sinoatrial ring
Stage Range:	Long-pec to Protruding-mouth

PHENOTYPE:

Fish:	sqet33mi28Et ; f2Tg + MO1-tnnt2a sqet33mi28Et ; npas4l^s5/s5 sqet33mi59BEt ; f2Tg + MO1-tnnt2a sqet33mi59BEt ; npas4l^s5/s5
Knockdown Reagent:	MO1-tnnt2a
Observed In:	cardiac conduction system sinoatrial node
Stage Range:	Long-pec to Protruding-mouth

Fig. 4

Loss of endocardium and hemodynamic stimulation affects development of the CCS. A: 3 dpf CCS:clo sibling. B: 3 dpf CCS:clo^m3^9-^/^- mutant. SAN CCS cells (arrows) spread widely in clo^m3^9-^/^-. C, E: Control CCS embryos at 2 dpf and 3 dpf, respectively. D, F: tnnt2 morphants at 2 dpf and 3 dpf, respectively. E′, F′: The same embryos as in E and F, showing dsRed expression of CCS:Tg(myl7:dsRed). Dotted white lines outline the chambers of the embryonic zebrafish heart. A-D: lateral view; E-H: ventral view. Abbreviations: A, atrium; SAN, sino-atrial region; V, ventricle.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
DsRed2	sqet33mi28Et ; f2Tg + MO1-tnnt2a	standard conditions	Protruding-mouth	myocardium	IFL
	sqet33mi28Et ; npas4l^s5/s5	standard conditions	Protruding-mouth	myocardium	IFL
	sqet33mi59BEt ; f2Tg	standard conditions	Protruding-mouth	myocardium	IFL
	sqet33mi59BEt ; f2Tg + MO1-tnnt2a	standard conditions	Protruding-mouth	myocardium	IFL
EGFP	sqet33mi28Et	standard conditions	Protruding-mouth	sinoatrial node	IFL
	sqet33mi28Et ; f2Tg	standard conditions	Long-pec	sinoatrial ring	IFL
	sqet33mi28Et ; f2Tg + MO1-tnnt2a	standard conditions	Long-pec	sinoatrial ring	IFL
			Protruding-mouth	sinoatrial node	IFL
	sqet33mi28Et ; npas4l^s5/s5	standard conditions	Protruding-mouth	sinoatrial node	IFL
	sqet33mi59BEt	standard conditions	Protruding-mouth	sinoatrial node	IFL
	sqet33mi59BEt ; f2Tg	standard conditions	Long-pec	sinoatrial ring	IFL
	sqet33mi59BEt ; f2Tg + MO1-tnnt2a	standard conditions	Long-pec	sinoatrial ring	IFL
			Protruding-mouth	sinoatrial node	IFL
	sqet33mi59BEt ; npas4l^s5/s5	standard conditions	Protruding-mouth	sinoatrial node	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
sqet33mi28Et ; f2Tg + MO1-tnnt2a	standard conditions	Long-pec	cardiac conduction system position, normal
		Long-pec	sinoatrial node position, normal
		Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal
sqet33mi28Et ; npas4l^s5/s5	standard conditions	Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal
sqet33mi59BEt ; f2Tg + MO1-tnnt2a	standard conditions	Long-pec	cardiac conduction system position, normal
		Long-pec	sinoatrial node position, normal
		Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal
sqet33mi59BEt ; npas4l^s5/s5	standard conditions	Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from Gene expression patterns : GEP, 21(2), Poon, K.L., Liebling, M., Kondrychyn, I., Brand, T., Korzh, V., Development of the cardiac conduction system in zebrafish, 89-96, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns

Acknowledgments:

ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 21(2), Poon, K.L., Liebling, M., Kondrychyn, I., Brand, T., Korzh, V., Development of the cardiac conduction system in zebrafish, 89-96, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns