ZFIN Figure: Poon et al., 2016, Fig. 4

ZFIN ID: ZDB-FIG-161101-4

ZFIN ID: ZDB-FIG-161101-4

Poon et al., 2016 - Development of the cardiac conduction system in zebrafish. Gene expression patterns : GEP 21(2):89-96 Full text @ Gene Expr. Patterns

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	DsRed2 EGFP
Fish:	sqet33mi28Et sqet33mi59BEt f2Tg; sqet33mi28Et f2Tg; sqet33mi59BEt f2Tg; sqet33mi28Et + MO1-tnnt2a f2Tg; sqet33mi59BEt + MO1-tnnt2a npas4l^s5/s5; sqet33mi28Et npas4l^s5/s5; sqet33mi59BEt
Knockdown Reagent:	MO1-tnnt2a
Anatomical Terms:	myocardium sinoatrial node sinoatrial ring
Stage Range:	Long-pec to Protruding-mouth

PHENOTYPE:

Fish:	f2Tg; sqet33mi28Et + MO1-tnnt2a f2Tg; sqet33mi59BEt + MO1-tnnt2a npas4l^s5/s5; sqet33mi28Et npas4l^s5/s5; sqet33mi59BEt
Knockdown Reagent:	MO1-tnnt2a
Observed In:	cardiac conduction system sinoatrial node
Stage Range:	Long-pec to Protruding-mouth

Fig. 4

Loss of endocardium and hemodynamic stimulation affects development of the CCS. A: 3 dpf CCS:clo sibling. B: 3 dpf CCS:clo^m3^9-^/^- mutant. SAN CCS cells (arrows) spread widely in clo^m3^9-^/^-. C, E: Control CCS embryos at 2 dpf and 3 dpf, respectively. D, F: tnnt2 morphants at 2 dpf and 3 dpf, respectively. E′, F′: The same embryos as in E and F, showing dsRed expression of CCS:Tg(myl7:dsRed). Dotted white lines outline the chambers of the embryonic zebrafish heart. A-D: lateral view; E-H: ventral view. Abbreviations: A, atrium; SAN, sino-atrial region; V, ventricle.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
DsRed2	f2Tg; sqet33mi28Et + MO1-tnnt2a	standard conditions	Protruding-mouth	myocardium	IFL
	f2Tg; sqet33mi59BEt	standard conditions	Protruding-mouth	myocardium	IFL
	f2Tg; sqet33mi59BEt + MO1-tnnt2a	standard conditions	Protruding-mouth	myocardium	IFL
	npas4l^s5/s5; sqet33mi28Et	standard conditions	Protruding-mouth	myocardium	IFL
EGFP	f2Tg; sqet33mi28Et	standard conditions	Long-pec	sinoatrial ring	IFL
	f2Tg; sqet33mi28Et + MO1-tnnt2a	standard conditions	Long-pec	sinoatrial ring	IFL
			Protruding-mouth	sinoatrial node	IFL
	f2Tg; sqet33mi59BEt	standard conditions	Long-pec	sinoatrial ring	IFL
	f2Tg; sqet33mi59BEt + MO1-tnnt2a	standard conditions	Long-pec	sinoatrial ring	IFL
			Protruding-mouth	sinoatrial node	IFL
	npas4l^s5/s5; sqet33mi28Et	standard conditions	Protruding-mouth	sinoatrial node	IFL
	npas4l^s5/s5; sqet33mi59BEt	standard conditions	Protruding-mouth	sinoatrial node	IFL
	sqet33mi28Et	standard conditions	Protruding-mouth	sinoatrial node	IFL
	sqet33mi59BEt	standard conditions	Protruding-mouth	sinoatrial node	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
f2Tg; sqet33mi28Et + MO1-tnnt2a	standard conditions	Long-pec	cardiac conduction system position, normal
		Long-pec	sinoatrial node position, normal
		Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal
f2Tg; sqet33mi59BEt + MO1-tnnt2a	standard conditions	Long-pec	cardiac conduction system position, normal
		Long-pec	sinoatrial node position, normal
		Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal
npas4l^s5/s5; sqet33mi28Et	standard conditions	Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal
npas4l^s5/s5; sqet33mi59BEt	standard conditions	Protruding-mouth	cardiac conduction system mislocalised, abnormal
		Protruding-mouth	sinoatrial node EGFP expression increased distribution, abnormal
		Protruding-mouth	sinoatrial node increased area, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Gene expression patterns : GEP, 21(2), Poon, K.L., Liebling, M., Kondrychyn, I., Brand, T., Korzh, V., Development of the cardiac conduction system in zebrafish, 89-96, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene expression patterns : GEP, 21(2), Poon, K.L., Liebling, M., Kondrychyn, I., Brand, T., Korzh, V., Development of the cardiac conduction system in zebrafish, 89-96, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns