ZFIN ID: ZDB-FIG-160929-10
Jung et al., 2016 - Impaired Lymphocytes Development and Xenotransplantation of Gastrointestinal Tumor Cells in Prkdc-Null SCID Zebrafish Model. Neoplasia (New York, N.Y.)   18:468-79 Full text @ Neoplasia
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

IF and RT-PCR analyses for activated lymphocytes. (A) IF detected by CD2AP marker. The CD2AP-reactive lymphocytes are present in the peripheral blood from both SCID and control zebrafish, suggesting that the cells are either activated T lymphocytes or NK cells. DAPI staining was used to detect the blood cells. (B) Double IF detected by CD2AP (Cy2) and NKP46 (Cy3) markers. The NKP46 is a specific marker for NK cells, whereas the CD2AP marker is reactive to both T lymphocytes and NK cells. (C) RT-PCR analysis. Glyceraldehyde 3-phosphate dehydrogenase gene detection was used as control. Nonspecific cytotoxic cell receptor protein-1 and NK specific protein (NK cell triggering receptor) gene detections indicate NK cell. TCR V(D)JC (Vb12-Cb1) and immunoglobulin (IgVH1-Cm) gene transcript detections reveal V(D)J recombination and heavy-chain rearrangement. (D) Counted numbers of cells depending on CD2AP or NKP46 positivity. Green arrowhead, positive for CD2AP; red arrowhead, positive for NKP46; white arrowhead, positive for both CD2AP and NKP46; blue arrowhead, lymphocytes negative for both CD2AP and NKP46; yellow arrowhead; red blood cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nccrp1 prkdcyu27/+ standard conditions Adult hematopoietic system RTPCR
prkdcyu27/yu27 standard conditions Adult hematopoietic system RTPCR
nktr prkdcyu27/+ standard conditions Adult hematopoietic system RTPCR
prkdcyu27/yu27 standard conditions Adult hematopoietic system RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-NCR1 IHC prkdcyu27/+ standard conditions Adult blood lymphocyte
IHC Adult spleen lymphocyte
IHC prkdcyu27/yu27 standard conditions Adult blood lymphocyte
IHC Adult spleen lymphocyte
Ab1-cd2ap IHC prkdcyu27/+ standard conditions Adult blood lymphocyte
IHC Adult spleen lymphocyte
IHC prkdcyu27/yu27 standard conditions Adult blood lymphocyte
IHC Adult spleen lymphocyte
Phenotype Details
Fish Conditions Stage Phenotype
prkdcyu27/yu27 standard conditions Adult B cell activation arrested, abnormal
Adult immunoglobulin heavy chain V-D-J recombination arrested, abnormal
Adult natural killer cell present, normal
Adult T cell activation arrested, abnormal
Adult T cell receptor V(D)J recombination arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neoplasia (New York, N.Y.) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neoplasia