Fig. 6

lmx1bb is expressed by dI5 and V0v neurons and these cells form in normal numbers in lmx1bb mutants. Lateral view of zebrafish spinal cord at 48 h (a-f), anterior left, dorsal top. in situ hybridization for lbx1a in WT (a) and lmx1bb homozygous mutant (b) embryos. in situ hybridization for evx1 + evx2 (evx) in WT (c) and lmx1bb homozygous mutant (d) embryos. Immunohistochemistry for EGFP in Tg(evx1:EGFP) ^SU1 WT (e) and lmx1bb homozygous mutant (f) embryos. g Mean number of cells (y-axis) in WT embryos (white) and lmx1bb homozygous mutants (grey) expressing lbx1a, evx or Tg(evx1:EGFP) ^SU1 in spinal cord region adjacent to somites 6-10 (x-axis). Two independent experiments were conducted for (a-f). Cell count results were similar in each replicate. Data shown here (g) are average values of 6-10 embryos from the same experiment. Precise number of embryos counted and p values are provided in Table 4. Error bars indicate standard error of the mean. Scale bar = 50 µm (a-f)