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ZFIN ID: ZDB-FIG-160928-15
Sarmah et al., 2016 - Embryonic Ethanol Exposure Dysregulates BMP and Notch Signaling, Leading to Persistent Atrio-Ventricular Valve Defects in Zebrafish. PLoS One   11:e0161205 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7

High Notch activity was not restricted at the AVC endocardial cells in ethanol exposed embryos.

(A-H) Tg(TP1:mCherry);Tg(kdrl:EGFP) double transgenic embryos showed compact cluster of strong mCherry labeled cells at the AVC and weak mCherry labeled cells in the ventricular endocardial lining in control embryo at 50 hpf (A, B); ethanol treated embryos had a mix of strong and weak mCherry labeled cells in the endocardium and no cluster of strong mCherry labeled cell at the AVC (C-H); severely defective embryo exhibited weaker mCherry labeling relative to the control (G, H). (I-N) Tg(TP1:mCherry);Tg(myl7:GFP) double transgenic embryos showed compact clusters of strong mCherry labeled cells at the AVC and OFT at 72 hpf in control (I) and no clustering in ethanol treated embryos (K, M); green fluorescence in Tg(TP1:mCherry);Tg(myl7:GFP) embryos showed myocardium at 72 hpf (J, L, N). A: Atrium, V: Ventricle, Arrowheads: AVC, Arrows: OFT.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP jh11Tg ; twu34Tg control Protruding-mouth cardiac muscle cell IFL
jh11Tg ; twu34Tg chemical treatment by environment: ethanol Protruding-mouth atrioventricular canal IFL
Protruding-mouth bulbus arteriosus IFL
Protruding-mouth cardiac muscle cell IFL
GFP jh11Tg; la116Tg control Long-pec atrial endocardium IFL
Long-pec endocardium IFL
Long-pec ventricular endocardium IFL
jh11Tg; la116Tg chemical treatment by environment: ethanol Long-pec atrial endocardium IFL
Long-pec endocardium IFL
Long-pec ventricular endocardium IFL
mCherry jh11Tg ; twu34Tg control Protruding-mouth atrioventricular canal IFL
Protruding-mouth bulbus arteriosus IFL
jh11Tg ; twu34Tg chemical treatment by environment: ethanol Protruding-mouth cardiac ventricle IFL
jh11Tg; la116Tg control Long-pec atrioventricular canal IFL
Long-pec ventricular endocardium IFL
jh11Tg; la116Tg chemical treatment by environment: ethanol Long-pec Not Detected atrioventricular canal IFL
Long-pec ventricular endocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jh11Tg ; twu34Tg chemical treatment by environment: ethanol Protruding-mouth atrioventricular canal Notch signaling pathway decreased occurrence, abnormal
Protruding-mouth cardiac ventricle mCherry expression mislocalised, abnormal
Protruding-mouth cardiac ventricle Notch signaling pathway increased occurrence, abnormal
jh11Tg; la116Tg chemical treatment by environment: ethanol Long-pec atrioventricular canal mCherry expression absent, abnormal
Long-pec atrioventricular canal Notch signaling pathway decreased occurrence, abnormal
Long-pec cardiac ventricle Notch signaling pathway increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One