ZFIN ID: ZDB-FIG-160921-2
Hartig et al., 2016 - Cortisol-treated zebrafish embryos develop into pro-inflammatory adults with aberrant immune gene regulation. Biology Open   5(8):1134-41 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 1

Effects of cortisol treatment on zebrafish larval cortisol levels, glucocorticoid receptor activity and gene expression. (A) Typical larva at 5days post-fertilization (dpf). (B) Whole-body cortisol levels after exposure to varying doses of cortisol from 0-5dpf, measured by ELISA of larval extracts (30 larvae per sample, duplicate measurements of each sample; P=0.015 by one-way ANOVA; * indicates that the difference from untreated controls is statistically significant by post hoc Tukey′s test). (C) GFP fluorescence in the glucocorticoid receptor-responsive reporter line SR4G at 5dpf following treatment with the indicated concentrations of cortisol (20 larvae imaged per sample; P=7.29×10-11 by a one way ANOVA; * indicates that the difference from untreated controls is statistically significant by post hoc Tukey′s test). (D) Gene ontology biological process parent terms found by PANTHER to be significantly (P<0.05) overrepresented in the list of genes differentially expressed in 5day old embryos treated with 1µM cortisol. (E) RNA-seq measurements of three genes found to be upregulated in embryos treated (+) with 1µM cortisol, compared to untreated controls (-). (F) RNA-seq measurements of three genes found to be downregulated in 1µM cortisol-treated embryos (+) compared to untreated controls (-). Differences in expression between control and treatment groups shown in E and F are all statistically significant (P<0.05). Error bars in B and C depict standard error of the mean (s.e.m.).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
anxa1c AB control Day 5 whole organism OTHR
AB chemical treatment by environment: Hydrocortisone Sodium Succinate Day 5 whole organism OTHR
cyp2aa8 AB control Day 5 whole organism OTHR
AB chemical treatment by environment: Hydrocortisone Sodium Succinate Day 5 whole organism OTHR
irg1l AB control Day 5 whole organism OTHR
AB chemical treatment by environment: Hydrocortisone Sodium Succinate Day 5 whole organism OTHR
klf2b AB control Day 5 whole organism OTHR
AB chemical treatment by environment: Hydrocortisone Sodium Succinate Day 5 whole organism OTHR
mpeg1.1 AB control Day 5 whole organism OTHR
AB chemical treatment by environment: Hydrocortisone Sodium Succinate Day 5 whole organism OTHR
socs3a AB control Day 5 whole organism OTHR
AB chemical treatment by environment: Hydrocortisone Sodium Succinate Day 5 whole organism OTHR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: Hydrocortisone Sodium Succinate Day 5 whole organism anxa1c expression decreased amount, abnormal
Day 5 whole organism cyp2aa8 expression decreased amount, abnormal
Day 5 whole organism irg1l expression increased amount, abnormal
Day 5 whole organism klf2b expression decreased amount, abnormal
Day 5 whole organism mpeg1.1 expression increased amount, abnormal
Day 5 whole organism socs3a expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open