FIGURE

Fig. 2

ID

ZDB-FIG-160916-5

Publication

Shibata et al., 2016 - Fgf signalling controls diverse aspects of fin regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Generation of a mosaic fin by a blastema transplant. (A) The procedure of the blastema transplantation and mosaic analysis. Donor blastema cells were taken from the double-transgenic zebrafish of Tg(hsp70l:dnfgfr1) and Tg(Olactb:loxP-dsR2-loxP-EGFP); the latter transgene drives ubiquitous expression of DsRed2 (Yoshinari et al., 2012). The regenerated fins are a chimera of host cells and transplanted donor cells. (B) A whole-mount fluorescent view of the mosaic fin at 5days post-transplantation (dpt). The fin contains progenies of the transplanted cells expressing the dsRed2. Scale bar: 500�m. (C) A longitudinal tissue section of the mosaic fin at 5dpt stained with an antibody against Zns5, a marker of osteogenic cells (green), and DAPI (blue). The transplanted cells (red) mostly gave rise to mesenchymal cells (11 Zns5-positive cells out of 226 DsRed2-positive cells, counted from 12 sections, from independent transplantations in four different fish). Scale bar: 50�m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development