FIGURE

Fig. 6

ID

ZDB-FIG-160826-6

Publication

Talbot et al., 2016 - Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Late-p1 is severely affected in itga8 and fras1 single and double mutants. (A-D) Tissue sections labeled with anti-P63 (epithelial nuclei) and sox9a:EGFP (cartilage) at 72 hpf, and oriented as shown in A′. The late-forming outpocketing of pouch-1 (late-p1) is indicated with a yellow dotted line in wild type. However, late-p1 is absent in (B) itga8^b1161, (C) fras1^b1048, and (D) fras1^b1048;itga8^b1161 double mutants, leaving a large gap (blue line) between endoderm and ectoderm. (E) Symplectic cartilage length and ectoderm-endoderm gap distance were measured on confocal stacks of whole-mounted embryos expressing sox9a:GFP and labeled with anti-P63. Each measurement pair is plotted as a dot, with 95% density ellipses shown for each genotype.

Expression Data

Gene:	GFP
Antibody:	Ab1-tp63
Fish:	sox9a^zc81Tg (AB) fras1^b1048/b1048; sox9a^zc81Tg (AB) itga8^b1161/b1161; sox9a^zc81Tg (AB) fras1^b1048/b1048; itga8^b1161/b1161; sox9a^zc81Tg (AB)
Anatomical Terms:	epithelial cell nucleus pharyngeal arch cartilage
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fras1^b1048/b1048; sox9a^zc81Tg (AB) itga8^b1161/b1161; sox9a^zc81Tg (AB) fras1^b1048/b1048; itga8^b1161/b1161; sox9a^zc81Tg (AB)
Observed In:	pharyngeal ectoderm pharyngeal endoderm
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 416(1), Talbot, J.C., Nichols, J.T., Yan, Y.L., Leonard, I.F., BreMiller, R.A., Amacher, S.L., Postlethwait, J.H., Kimmel, C.B., Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction, 136-48, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.