FIGURE

Fig. 2

ID

ZDB-FIG-160819-20

Publication

Otten et al., 2016 - Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells

Other Figures

Fig. 2

All Figure Page

Back to All Figure Page

Fig. 2

The mtDNA Copy Number in FACS-Isolated PGCs and Non-PGCs from Zebrafish Embryos

(A) GFP expression in transgenic zebrafish embryos (EGFP-nanos3 3′ UTR). (A) Embryos were imaged using an Axioplan M1 Zeiss microscope. The images represent a combination of pictures captured along the anterior-posterior axis of the embryo, using specific focal planes. Scale bars represent 100 �m for 8 hpf and 200 �m for 24, 48, and 72 hpf.

(B and C) Absolute mtDNA copy number in (B) PGCs and (C) non-PGCs from various stages of development. Every symbol represents one group of 80 cells. Horizontal lines indicate mean with SD. The p values are generated by Spearman?s rank correlation test and indicate a downward trend when <0.05. R² indicates fitness of a non-linear one-phase decay exponential equation.

(D) Plot of the non-linear one-phase decay equation for PGCs (derived from the graph in B) and non-PGCs (derived from C). The half-life was 6.9 hpf for PGCs and 3.4 hpf for non-PGCs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.