Fig. 1

Identification of a cold-sensitive mutation in ntla. A-D: A cross from one family of fish produced embryos with a truncated tail phenotype (C, D) as well as wild-type (wt) embryos after being raised overnight at 17°C from shield stage. The phenotype is less severe than seen with the ntla^b195 mutant (B, ntla^-/-). E-H: Staining with the muscle antibody MF20 shows that the embryos with the truncated tails have less somitic tissue (G,H), although with less reduction than seen with ntla^-/- (F). I: The mutant embryos have two base pair changes in the C-terminal domain of the Ntla coding region. The numbers indicate the base pair where the change occurred relative to the first base of the start codon. J,K: A cross of ntla^-/+ and ntla^cs/+ adults produced phenotypically wild-type and mutant embryos after being raised overnight at 17°C from shield stage. The genotypes of both are indicated on the figure. All embryos are shown at the equivalent of two dpf.