Fig. 3

Expression markers for heart and skeletal muscle in smyd5 morphants by WISH and electron microscopic analysis of heart, skeletal muscle, and slow muscle.

WISH analysis of skeletal muscle and cardiac chamber markers at 12 (A), 24 (B), and 48 hpf (C). (D) Electron microscopic analysis of heat, skeletal muscle, and slow muscle at 48 hpf. (A) Expression of myf5, myod, myog, and gata5 in embryos injected with smyd5-MO1, control embryos injected with Con-MO, and those no-injection at 12 hpf. (B) Expression of myod, myog, and mck in embryos injected with smyd5-MO1, control embryos, and those no-injection at 24 hpf. (C) Expression of cmlc2 in morphants, control embryos, and those no-injection at 48 hpf. Embryos are shown in the dorsal view, anterior towards the left (A). Embryos are depicted in the lateral view (B) and in the frontal view, dorsal towards the left (C). (D) Electron micrographs of parasagittal sections through cardiac and somitic muscle cells of embryos injected with smyd5-MO1, and control embryos at 48 hpf. Numbers in the bottom of each panel indicate the number of embryos with the representative phenotype per the total number of examined embryos. Scale bar, 200 µm (black) and 1 µm (white). skm, skeletal muscle.