ZFIN ID: ZDB-FIG-160802-14
Tang et al., 2016 - Gene knockout of nuclear progesterone receptor provides insights into the regulation of ovulation by LH signaling in zebrafish. Scientific Reports   6:28545 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 5

Regulation of ovulation-related genes by LH signaling in npr+/+ and npr-/- fish.

(a) The timescale of hCG-induced oocyte maturation and ovulation in npr+/+ zebrafish. Follicles were isolated at different time points after hCG (10 IU/µl) injection. Matured follicle with transparent appearance is indicated by black arrow and ovulated oocyte with enlarged fertilization membrane is indicated by white arrow. (b) The expression profile of npr after hCG injection. (c) The expression profile of ovulation related genes in npr+/+ and npr/ fish after hCG injection. (i) Genes involved in prostaglandin biosynthesis; mmps and tissue inhibitors of metalloproteinases (timp2b); (iii) Genes involved in steroidogenesis; (iv) A disintegrin and metalloprotease with thrombospondin motifs1 (adamts1) and (v) cathepsin L (ctsl). The expression levels were normalized to that of ef1α and then to the expression level in npr+/+ fish at 0 h. Different letters in each dataset indicated statistical significance (P < 0.05). Each value represents the mean value ± SEM (n = 4) of three independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001 vs npr+/+).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
adamts1 WT chemical treatment by injection: Chorionic gonadotropin Adult ovarian follicle RTPCR
pgrzf723/zf723(AB) chemical treatment by injection: Chorionic gonadotropin Adult ovarian follicle RTPCR
ptger4b WT chemical treatment by injection: Chorionic gonadotropin Adult ovarian follicle RTPCR
pgrzf723/zf723(AB) chemical treatment by injection: Chorionic gonadotropin Adult ovarian follicle RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by injection: Chorionic gonadotropin Adult ovarian follicle adamts1 expression increased amount, abnormal
Adult ovarian follicle ptger4b expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.