Fig. S5
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- ZDB-FIG-160727-23
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- Kim et al., 2016 - Recruitment of Rod Photoreceptors from Short-Wavelength-Sensitive Cones during the Evolution of Nocturnal Vision in Mammals
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Absence of sws2 Opsin Expression History in Zebrafish Rod Photoreceptors, related to Figure 6. (A) sws2 opsin lineage tracing strategy in zebrafish. Lineage tracing was performed using a Kaloop feedforward system. Two constructs Tg[sws2:KalTA4] and Tg[UAS:nfsB-mCherry-V2A-KalTA4] (ua3138 and ua3137, respectively) were independently inserted to generate the transgenic zebrafish. In ua3137, a fluorescent reporter protein mCherry is fused in-frame to a bacterial nitroreductase gene (nfsB), and the fusion protein is connected via the labile linker peptide V2A to a second copy of the KalTA4 transcription factor. (B) Lineage tracing of sws2- expressing cells using sws2:KalTA4; UAS:nfsB-mCherry-V2A-KalTA4 zebrafish line. The UV-sensitive cones (cyan, 10C9.1) and rods (green, 4C12) of 4 dpf zebrafish were labeled using immunohistochemistry. Representative images are shown for 1 of the 10 left eyes. A vast majority of lineage-traced cells (4,273 of 4,278; 99.88%) clearly lack immunolabeling by 4C12 (a rod marker), whereas 5 of the cells (0.12%) showed somewhat ambiguous (unclear detection) labeling. D, dorsal; V, ventral; N, nasal; T, temporal. Scale bar: 50 µm in low magnification images, and 20 µm in the high magnification image. |
Reprinted from Developmental Cell, 37, Kim, J.W., Yang, H.J., Oel, A.P., Brooks, M.J., Jia, L., Plachetzki, D.C., Li, W., Allison, W.T., Swaroop, A., Recruitment of Rod Photoreceptors from Short-Wavelength-Sensitive Cones during the Evolution of Nocturnal Vision in Mammals, 520-532, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell