FIGURE

Fig. S5

ID

ZDB-FIG-160714-26

Publication

Richardson et al., 2016 - Re-epithelialization of cutaneous wounds in adult zebrafish uses a combination of mechanisms at play during wound closure in embryonic and adult mammals

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

Efficacy of SB-431542 (TGFβ) and SP600125 (JNK) inhibitors. (A,B) Live images of embryos treated with 0.1% DMSO (A, control) or with 10 µM SB431542 (B), starting at 4 hours post fertilization (hpf); 24 hpf, lateral view. The SB431542-treated embryo displays fused eyes (arrowhead) and lack of trunk somites (arrow), resembling the phenotype of mutants lacking the TGFβ family members Ndr1 and Ndr2 (Feldman et al., 1999), indicating that 10 µM SB431542 efficiently blocks TGFβ signalling (n=45/48; 2 independent experiments). (C) Duplex anti-pJNK (1:1000, Cell Signaling Technology, 4668s) and anti-&beta-actin (loading control; 1:1000, Sigma-Aldrich, A2228) Western blot of protein extracts from fins of adult zebrafish, after treatment with 0.1% DMSO (control) or 10 µM SP600125 for 5 hours, and separation via 9% SDS-PAGE. Levels of the phosphorylated version of the p54 splice isoform of JNK are several-fold reduced in the SP600125-treated sample. Similar results were obtained in three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development