FIGURE

Fig. 3

ID

ZDB-FIG-160701-11

Publication

Tong et al., 2016 - The FBPase Encoding Gene glpX Is Required for Gluconeogenesis, Bacterial Proliferation and Division In Vivo of Mycobacterium marinum

Other Figures

Fig. 3

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Fig. 3

M.marinum ΔglpX is attenuated in zebrafish.

(A) Survival curves of adult zebrafish during a 21-day infection course. Fish were intraperitoneally infected at an initial dosage of 10⁴ CFU/fish of WT, ΔglpX, and complemented strain ΔglpX/C (glpX). (B) Zebrafish larvae were infected with WT (56 CFU), ΔglpX (168 CFU) and complemented strain ΔglpX/C (glpX) (26 CFU) carrying pTEC15 containing fluorescent protein Wasabi separately. Representative fluorescent images of infected larvae at 5 dpi. The arrow points to the formation of initial granuloma with fluorescent M. marinum inside. Bacterial burdens of infected larvae were determined by FPC at 4 dpi (C) and 6 dpi (D), OD = lg^{255/PixelValue}, P values are calculated by an unpaired Student t test. (E) The relative transcription of inflammatory cytokines were measured by qRT-PCR using total RNA extracted from zebrafish. 0.01<*P≤0.05, 0.001<**P ≤0.01, ***P ≤0.001 by Kruskal-Wallis test and Dunn′s Multiple Comparison Test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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