FIGURE SUMMARY
Title

The FBPase Encoding Gene glpX Is Required for Gluconeogenesis, Bacterial Proliferation and Division In Vivo of Mycobacterium marinum

Authors
Tong, J., Meng, L., Wang, X., Liu, L., Lyu, L., Wang, C., Li, Y., Gao, Q., Yang, C., Niu, C.
Source
Full text @ PLoS One
Fig. 3

M.marinum ΔglpX is attenuated in zebrafish.

(A) Survival curves of adult zebrafish during a 21-day infection course. Fish were intraperitoneally infected at an initial dosage of 104 CFU/fish of WT, ΔglpX, and complemented strain ΔglpX/C (glpX). (B) Zebrafish larvae were infected with WT (56 CFU), ΔglpX (168 CFU) and complemented strain ΔglpX/C (glpX) (26 CFU) carrying pTEC15 containing fluorescent protein Wasabi separately. Representative fluorescent images of infected larvae at 5 dpi. The arrow points to the formation of initial granuloma with fluorescent M. marinum inside. Bacterial burdens of infected larvae were determined by FPC at 4 dpi (C) and 6 dpi (D), OD = lg255/PixelValue, P values are calculated by an unpaired Student t test. (E) The relative transcription of inflammatory cytokines were measured by qRT-PCR using total RNA extracted from zebrafish. 0.01<*P≤0.05, 0.001<**P ≤0.01, ***P ≤0.001 by Kruskal-Wallis test and Dunn′s Multiple Comparison Test.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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