Fig. 4

In vivo modelling reveals broader developmental defects and underdeveloped cataractous lenses in dnajb1a morphants.

dnajb1a zebrafish morphants recapitulate PA symptoms. Dorsal view of (a) control morphant and (b) dnajb1a morphant embryos suggest a relatively compact lens observed at 4 days post fertilization as a result of dnajb1a MO. Lateral view of (c) control morphant and (d) dnajb1a morphant embryos showing broader eye developmental defects including underdeveloped cataractous lenses in dnajb1a MO-injected embryos. (e) Quantification of the compact lens phenotype in the dnajb1a, hspa4l and kif3a morphants and rescue of morphant phenotype with zebrafish orthologue of dnajb1a mRNA. Note: kif3a MO did not show any ocular phenotype; however, the morphants exhibited morphological and developmental defects, such as body curvature, consistent with the ciliary phenotypes. Lateral view of (f) control morphant and (g) dnajb1b morphant embryos illustrate a relatively compact cataractous lenses observed at 4 days post fertilization as a result of dnajb1b translation-blocking MO.