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Fig. 6

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ZDB-FIG-160607-9

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Enyedi et al., 2016 - The Cell Nucleus Serves as a Mechanotransducer of Tissue Damage-Induced Inflammation

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Fig. 6

Swollen Nuclei that Contain cPla₂ Attract Leukocytes to Cell Corpses

(A) Left: scheme of experimental design. Amputated zebrafish larvae were immersed in isotonic bathing solution to inhibit endogenous, wound-induced inflammation. Amputation wounds were exposed to differently conditioned HeLa cell suspensions. Nuclear swelling was initiated by digitonin-permeabilization and blocked by PVP-supplementation as described. Right: average number of leukocytes recruited to the wound margin within 60 min after cell-exposure of amputated tail fins at indicated conditions (table). Note that only swollen (condition 5), but not nonswollen nuclei (conditions 3 and 4) that contain cPla₂-mK2 attract leukocytes to cell corpses at permissive [Ca²⁺]. Error bars, SEM. ***p < 0.0005, t test. n, number of animals.

(B) Transmitted light images of zebrafish larvae exposed to permeabilized cell corpses with nonswollen (top, condition 4) or swollen (bottom, condition 5) nuclei that contain cPla₂-mK2. Representative leukocyte tracks are superimposed and color-coded. Please refer to the Experimental Procedures for details. Scale bars, 100 µm.

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Reprinted from Cell, 165, Enyedi, B., Jelcic, M., Niethammer, P., The Cell Nucleus Serves as a Mechanotransducer of Tissue Damage-Induced Inflammation, 1160-1170, Copyright (2016) with permission from Elsevier. Full text @ Cell