FIGURE

Fig. S2

ID

ZDB-FIG-160525-29

Publication

Zhao et al., 2016 - An essential role for Grk2 in Hedgehog signalling downstream of Smoothened

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Fig. S2

Phosphorylation and dimerisation of Smo does not impair or enhance Hh activity

A. Western blot analysis of wildtype and mutant forms of Smo expressed from bicistronic myctagged mSmo and GFP constructs injected into wildtype zebrafish embryos. The histogram shows the quantification of Myctagged mSmo vs. GFP (n = 3). The error bars indicate SD.

B. Normalised expression of ptch2 in 18hpf wildtype (AB), Cyc(A)treated embryos and embryos injected with mRNA encoding Shh or various Smo mutants. Individual data points (blue crosses) are shown; mean is indicated by thick solid line, and SD by thin bars. Unpaired Student′s ttest was used to determine the statistical significance between control (AB) and experimental embryos. ***P < 0.001; **P < 0.01; and n.s. (not significant).

C. Prox1a and Eng expression in smo^hi1640 mutant embryos injected with mRNA encoding mSmoSD (n = 7) and mSmoKRA (n = 7). These mutant embryos show full recovery of SSFs and MPs. Embryos shown are at 30hpf. Scale bar, 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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