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ZFIN ID: ZDB-FIG-160426-6
Berg et al., 2016 - Lysosomal Disorders Drive Susceptibility to Tuberculosis by Compromising Macrophage Migration. Cell   165:139-152 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 5

Lysosomal Storage Disorders Disrupt Macrophage Migration and Cause Granuloma Breakdown

(A and B) Confocal images of green fluorescent macrophages in the brain of 3-dpf control and morphant larvae, unstained (A) or following staining with LysoTracker Red (B). Scale bars, 10 µm.

(C) Quantification of average macrophage speed in control and morphant larvae by macrophage morphology (wt, wild-type; vac, vacuolated).

(D-F) Quantification of bacterial cording in control and morphant larvae at 3 dpi with 200 Mm. Statistical significance was determined using paired t tests with Bonferroni correction (C) and Fisher’s exact test (D-F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
YFP w200Tg + MO1-arsa(AB) control Protruding-mouth macrophage IFL
w200Tg + MO1-hexa(AB) control Protruding-mouth macrophage IFL
w200Tg + MO3-gba(AB) control Protruding-mouth macrophage IFL
w200Tg(AB) control Protruding-mouth macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w200Tg + MO1-arsa(AB) control Protruding-mouth macrophage lysosome accumulation macrophage, abnormal
Protruding-mouth macrophage morphology, abnormal
Protruding-mouth macrophage myeloid leukocyte migration decreased process quality, abnormal
Protruding-mouth macrophage myeloid leukocyte migration process quality, abnormal
w200Tg + MO1-arsa(AB) bacterial treatment by injection: Mycobacterium marinum Day 4 - Day 6 response to bacterium decreased sensitivity of a process response to bacterium, abnormal
w200Tg + MO1-hexa(AB) control Protruding-mouth macrophage lysosome accumulation macrophage, abnormal
Protruding-mouth macrophage morphology, abnormal
Protruding-mouth macrophage myeloid leukocyte migration decreased process quality, abnormal
Protruding-mouth macrophage myeloid leukocyte migration process quality, abnormal
w200Tg + MO1-hexa(AB) bacterial treatment by injection: Mycobacterium marinum Day 4 - Day 6 response to bacterium decreased sensitivity of a process response to bacterium, abnormal
w200Tg + MO3-gba(AB) control Protruding-mouth macrophage lysosome accumulation macrophage, abnormal
Protruding-mouth macrophage morphology, abnormal
Protruding-mouth macrophage myeloid leukocyte migration decreased process quality, abnormal
Protruding-mouth macrophage myeloid leukocyte migration process quality, abnormal
w200Tg + MO3-gba(AB) bacterial treatment by injection: Mycobacterium marinum Day 4 - Day 6 response to bacterium decreased sensitivity of a process response to bacterium, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 165, Berg, R.D., Levitte, S., O'Sullivan, M.P., O'Leary, S.M., Cambier, C.J., Cameron, J., Takaki, K.K., Moens, C.B., Tobin, D.M., Keane, J., Ramakrishnan, L., Lysosomal Disorders Drive Susceptibility to Tuberculosis by Compromising Macrophage Migration, 139-152, Copyright (2016) with permission from Elsevier. Full text @ Cell