FIGURE

Fig. 6

ID

ZDB-FIG-160407-10

Publication

Shiu et al., 2016 - Knockdown of zebrafish YY1a can downregulate the phosphatidylserine (PS) receptor expression, leading to induce the abnormal brain and heart development

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Fig. 6

Morpholino-induced knockdown of YY1a results in brain and heart defects. Morphological analysis of embryos injected with either 25 ng of control-MO or YY1-MO and examined at 48 hpf or 3 dpf following staining with pax 2a, nkx 2.5, or no stain. a Panels a-c. The embryos are stained with pax 2a (a, b; top view, anterior to the right) or not stained (d-f; lateral view). The YY1-MO-injected embryos (panel c) has smaller brain (indicated by black arrow) and abnormal pax 2a pattern (indicated by red arrow) as compared with wild type embryos (panel a) and control-MO-injected embryos (panel b). The fore-, mid-, and hindbrains are shorter (indicated by open square [panel f]; cf. with control [panels d and e]). b Staining with nkx 2.5 was used to monitor heart development. Normal heart formation was delayed at 48 hpf (panel c; indicated by star). Compare with the atria (A) and ventricles (V) in panels a and b. The tube-like heart in panel f (indicated by arrows) should be compared with that in wild type and control-MO-injected embryos (panels d and e, respectively) at 3 dpf. Bars indicate 100 µm

Expression Data

Genes:	nkx2.5 pax2a
Fish:	WT WT + MO1-yy1a
Knockdown Reagent:	MO1-yy1a
Anatomical Terms:	atrium brain cardiac ventricle heart
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-yy1a
Knockdown Reagent:	MO1-yy1a
Observed In:	atrium blood circulation brain forebrain heart heart development hindbrain midbrain whole organism anterior-posterior axis
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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