Fig. 2

Ontogenetic pattern of thraa and thrb expression in zebrafish. Panel A) qRT-PCR of thraa and thrb absolute mRNAs expression during embryonic development and larval transition (1-120hpf). The average expression of thraa and thrb during the zygote-blastula stage (1-4 hpf), gastrula-segmentation (6-18 hpf) and late embryonic and larval stages (24-120 hpf) are illustrated in the inset. **p < 0.01; ***p < 0.001; ###p < 0.001. Panel B) qRT-PCR of thraa and thrb absolute expression in adult tissues, total RNA was extracted from 5 adults. *p < 0.05; ***p < 0.001. Panels C-J) Tissue-specific expression of TRs, at different developmental stages, analyzed by WISH using thraa or thrb riboprobes followed by NBT/BCIP staining. Images are representative of 3 experiments (50 embryos each). ap: animal pole of the zygote; cns: central nervous system; pd: pronephric ducts; h: heart; pf: pectoral fins; anc: anterior neuromast cells; pnc: posterior neuromast cells; git: gastrointestinal tract; jc: jaw cartilages; sb: swim bladder; e: eyes, pi: pituitary; ov: otic vesicle.