FIGURE

Fig. 3

ID

ZDB-FIG-160317-11

Publication

Wu et al., 2016 - Multiple signaling factors and drugs alleviate neuronal death induced by expression of human and zebrafish tau proteins in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Zebrafish Nrf2 overexpression prevented human 4R-tau-GFP and zebrafish 3R-tau-GFP induced Neuronal death. a GFP-labeled neuronal cells and axons were observed at 24 and 48 hpf in embryos co-injected with pHuC-zNrf2-HA-2A-mCherry and pHuC-z3R-tau-GFP (b) or pHuC-h4R-tau-GFP (d). For comparison, embryos co-injected with pHuC-mCherry (panels a and c) were used as the control. The boxed regions are enlarged (a′-d′′) to show the GFP-labeled neuronal cells in 24 and 48 hpf embryos from the lateral view. Scale bars: 100 µm. The protection effect of zNrf2 against neuronal death induced by human tau-GFP or zebrafish tau-GFP was presented in panel e to show higher percentage, 58 % and 55 % of zebrafish embryos expressing zNrf2 with more neuronal cells, compared to 19 % and 17 % without zNrf2. b GFP signals (panel a) and mCherry signals (panel a′) in neuronal cells and axons in embryos co-injected with pHuC-h4R-tau-GFP and pHuC-zNrf2-HA-2A-mCherry were colocalized (panel a′′). Scale bar: 50 µm. c Double immunostaining of h4R-tau-GFP (GFP antibody) and zNrf2-HA (HA antibody, panel a′) in spinal cord neurons of the aforementioned zebrafish embryos. The phosphorylation state of h4R-tau-GFP was detected using antibody pT212 (panel b) and antibody AT8 (panel b′). Scale bar: 50 µm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.