FIGURE

Fig. 1

ID

ZDB-FIG-160304-62

Publication

McPherson et al., 2016 - Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

A th2:GFP Transgene Labels Dopaminergic Neurons Derived from a dlx5/6⁺ Precursor Lineage

(A) Live zebrafish 7 dpf larva expressing th2:GFP with schematic of th2 enhancer/promoter region used for transgenes.

(B) Ventral whole-mount view of th2:GFP expression in a dissected 7 dpf brain; box indicates area of posterior recess shown in (C)?(F).

(C) Simultaneous in situ hybridization for th2 mRNA (left) and anti-GFP immunohistochemistry (middle) shows transcript expression in all th2:GFP⁺ cells (right). Ventral whole-mount view of a dissected 7 dpf brain is shown.

(D) Co-expression analysis shows that most th2:GFP⁺ cells (green) express dlx5/6:mCherry (magenta), indicating their origin from dlx5/6⁺ precursors.

(E) Co-expression analysis shows that most th2:GFP⁺ cells (green) express dopamine (magenta).

(F) Co-expression analysis shows that few th2:GFP⁺ cells (green) express serotonin (magenta).

All images in (D)?(F) are ventral maximum intensity confocal z projections of the hypothalamic posterior recess from dissected 7 dpf brains. Individual and merged channels of boxed regions are shown in the lower panels. The scale bar in (B) represents 50 �m; all other scale bars represent 10 �M.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.