PUBLICATION
Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation
- Authors
- McPherson, A.D., Barrios, J.P., Luks-Morgan, S.J., Manfredi, J.P., Bonkowsky, J.L., Douglass, A.D., Dorsky, R.I.
- ID
- ZDB-PUB-160118-1
- Date
- 2016
- Source
- Current biology : CB 26(2): 263-9 (Journal)
- Registered Authors
- Bonkowsky, Joshua, Dorsky, Richard, McPherson, Adam D.
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Behavior, Animal/physiology
- Dopamine/metabolism
- Dopaminergic Neurons/metabolism*
- Hypothalamus/metabolism*
- Motor Activity/physiology*
- Neurogenesis/physiology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism*
- PubMed
- 26774784 Full text @ Curr. Biol.
Citation
McPherson, A.D., Barrios, J.P., Luks-Morgan, S.J., Manfredi, J.P., Bonkowsky, J.L., Douglass, A.D., Dorsky, R.I. (2016) Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation. Current biology : CB. 26(2):263-9.
Abstract
Postembryonic neurogenesis has been observed in several regions of the vertebrate brain, including the dentate gyrus and rostral migratory stream in mammals, and is required for normal behavior [1-3]. Recently, the hypothalamus has also been shown to undergo continuous neurogenesis as a way to mediate energy balance [4-10]. As the hypothalamus regulates multiple functional outputs, it is likely that additional behaviors may be affected by postembryonic neurogenesis in this brain structure. Here, we have identified a progenitor population in the zebrafish hypothalamus that continuously generates neurons that express tyrosine hydroxylase 2 (th2). We develop and use novel transgenic tools to characterize the lineage of th2(+) cells and demonstrate that they are dopaminergic. Through genetic ablation and optogenetic activation, we then show that th2(+) neurons modulate the initiation of swimming behavior in zebrafish larvae. Finally, we find that the generation of new th2(+) neurons following ablation correlates with restoration of normal behavior. This work thus identifies for the first time a population of dopaminergic neurons that regulates motor behavior capable of functional recovery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping