FIGURE

Fig. 3

ID

ZDB-FIG-160303-38

Publication

Stegmaier et al., 2016 - Real-Time Three-Dimensional Cell Segmentation in Large-Scale Microscopy Data of Developing Embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Whole-Embryo Cell Segmentation in Fruit Fly, Zebrafish, and Mouse

(A) Maximum-intensity projections of 3D image datasets of fruit fly, zebrafish, and mouse embryos expressing fluorescent markers labeling all membranes. Image data were acquired with light-sheet fluorescence microscopy (Tomer et al., 2012). The fruit fly embryo was imaged at 3 hr after egg laying (AEL), the zebrafish embryo at 6 hr post fertilization (hpf), and the mouse embryo at E6.5 (Experimental Procedures). Scale bar, 50 µm.

(B) Segmentation results, visualized as renderings of sliced embryos. Cells in the exposed cross-sections are shown in an orange/red color scheme, and in a cyan/blue color scheme for the rest of the embryo. Insets show enlarged views of the cell segmentation results.

(C) As in (A), but for fruit fly, zebrafish, and mouse embryos imaged with confocal fluorescence microscopy (Zeiss LSM 510 and LSM 710 microscopes). The fruit fly embryo was imaged at 3 hr AEL, the zebrafish embryo at 6 hpf, and the mouse embryo at E7.5 (Experimental Procedures). Scale bar, 50 µm.

(D) Segmentation results for the image data shown in (C).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 36, Stegmaier, J., Amat, F., Lemon, W.C., McDole, K., Wan, Y., Teodoro, G., Mikut, R., Keller, P.J., Real-Time Three-Dimensional Cell Segmentation in Large-Scale Microscopy Data of Developing Embryos, 225-240, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell