Fig. 7
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- ZDB-FIG-160226-7
- Publication
- Miyagi et al., 2016 - Characterization of the zebrafish cx36.7 gene promoter: Its regulation of cardiac-specific expression and skeletal muscle-specific repression
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EGFP expression in transgenic embryos using the 316- or 133-bp promoter constructs. (A and B) One-cell stage fertilized eggs were injected with the 316- (A) or 133-bp (B and C) promoter constructs. At 2 dpf, EGFP expression was observed. Typical images of embryos with EGFP expression in the heart (arrows) and trunk muscle (arrowheads) are shown. (C) A high magnification image of the trunk region of the embryo in B. (D) An image of a control embryo without injection is shown. Zebrafish embryos exhibit high autofluorescence in the yolk sac (asterisk). (E and F) EGFP expression of 2-dpf stable zebrafish lines carrying the 500- (E) or 133-bp (F) promoter constructs. EGFP signal in the heart and trunk muscle is indicated by arrows and arrowheads, respectively. (G) An image of a control non-transgenic embryo is shown. Bars, 500 µm (A, B, and D–G) and 200 µm (C). |
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Reprinted from Gene, 577(2), Miyagi, H., Nag, K., Sultana, N., Munakata, K., Hirose, S., Nakamura, N., Characterization of the zebrafish cx36.7 gene promoter: Its regulation of cardiac-specific expression and skeletal muscle-specific repression, 265-74, Copyright (2016) with permission from Elsevier. Full text @ Gene