Fig. 2

il1b expression in tlr2 and tlr5 morphants following PAMPs stimulation. Embryos were injected at the 1-2 cells stage with standard control (Sc), tlr2, tlr5a or tlr5b morpholino (MO) and subsequently injected with Pam3CSK4 at 27 hpf, flagellin or water as a mock control. Expression of il1b was determined by qPCR at 1 hpi. a tlr5a and tlr5b knockdown effect on il1b RNA expression in response to flagellin. b C tlr2 knockdown effect on il1b RNA expression in response to Pam3CSK4 (b) or flagellin (c). Data (mean ± SEM) are combined from at least three biological replicates (n = 10 embryos per group) and expressed relative to their corresponding water control, which is set at 1. Statistical significance was determined by ANOVA analysis and Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001; Pam, Pam3CSK4 injecton; Fla, flagellin injection