FIGURE

Fig. 1 S1

ID

ZDB-FIG-160217-11

Publication

Yokota et al., 2015 - Endothelial Ca(2+) oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

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Fig. 1 S1

GCaMP7a works as a Ca²⁺ indicator in endothelial cells (ECs).

(A) HUVECs transfected with GCaMP7a expression plasmids were treated with DMSO (upper) or ionomycin (lower). GCaMP7a images before (-) and after the treatment (40 s) are shown. (B) Confocal stack fluorescence images of Tg(fli1:Gal4FF);(UAS:GCaMP7a) embryos at 26 ss treated with DMSO (upper) or 25 µM ionomycin (lower). GCaMP7a images before (-) and after the treatment (30 min) are shown. (C) Fluorescence images of HUVECs transfected with GCaMP7a expression plasmids pretreated with DMSO or 25 µM BAPTA-AM for 30 min and treated with 50 ng/ml VEGF-A before (-) and after the treatment (100 s). Note that enhancement of GCaMP7a fluorescence by VEGF-A is blocked by pretreatment with BAPTA-AM. Scale bars, 10 µm in A-C.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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